Cross-linked enzyme aggregates (CLEAs) of selected lipases: a procedure for the proper calculation of their recovered activity
نویسندگان
چکیده
منابع مشابه
Use of Cross-Linked Enzyme Aggregates (CLEAs) for performing biotransformations
The methodology for the preparation of cross-linked enzyme aggregates (CLEAs) and their application as highly effective, recyclable biocatalysts in a wide variety of transformations is reviewed. The method constitutes a combination of enzyme purification and immobilization into a single operation and allows for the use of relatively crude enzyme preparations. The resulting CLEAs have excellent ...
متن کاملCross-linked enzyme aggregates (CLEAs): stable and recyclable biocatalysts.
The key to obtaining an optimum performance of an enzyme is often a question of devising an effective method for its immobilization. In the present review, we describe a novel, versatile and effective methodology for enzyme immobilization as CLEAs (cross-linked enzyme aggregates). The method is exquisitely simple (involving precipitation of the enzyme from aqueous buffer followed by cross-linki...
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ژورنال
عنوان ژورنال: AMB Express
سال: 2013
ISSN: 2191-0855
DOI: 10.1186/2191-0855-3-25